polyclonal rabbit anti human fibrinogen  (Valiant Co Ltd)

Journal: Toxins

Article Title: Fibrinogenolysis in Venom-Induced Consumption Coagulopathy after Viperidae Snakebites: A Pilot Study

doi: 10.3390/toxins14080538

Figure Lengend Snippet: Results of western blot analysis of patient’s plasma proteins after snakebite, over time. Plasma proteins were separated by SDS-PAGE under reduced conditions followed by western blot analysis using polyclonal anti-human fibrinogen antibodies (DAKO, HPST, Prague, Czech Republic). Western blot analysis of human fibrinogen in patients’ plasma after bites by Atheris nitschei ( a ), Calloselasma rhodostoma ( b ), Crotalus adamenteus case 1 ( c ). MW—protein molecular weight marker (Thermo Scientific TM , Spectra TM Multicolor Broad Range Protein Ladder, Life Technologies Czech Republic Ltd., Prague, Czech Republic). Molecular masses (kDa) are indicated on the left side. FBG—human fibrinogen standard. The sample number is provided in the top line and hours since bite in the bottom line. Red arrows mark fibrinogen fragments induced by snakebite. The chains of human fibrinogen standards are highlighted with red square boxes. Note: Samples 1 and 2 in ( a ) are missing. They were not analyzed by Western blot.

Article Snippet: The FBG immunodetection was performed using rabbit anti-human fibrinogen polyclonal antibody (DAKO, HPST, Prague, Czech Republic; dilution 1:50,000 in 0.1% Tween/Tris-buffered saline) for 1 h at room temperature.

Techniques: Western Blot, SDS Page, Molecular Weight, Marker

Journal: Toxins

Article Title: Fibrinogenolysis in Venom-Induced Consumption Coagulopathy after Viperidae Snakebites: A Pilot Study

doi: 10.3390/toxins14080538

Figure Lengend Snippet: Results of western blot analysis of patients’ plasma proteins after snakebite over time. Plasma proteins were separated by SDS-PAGE under reduced conditions followed by western blot analysis using polyclonal anti-human fibrinogen antibodies (DAKO, HPST, Prague, Czech Republic). Western blot analysis of human fibrinogen in patients’ plasma after bites by Crotalus adamanteus case 2 ( a ), Echis coloratus ( b ), Macrovipera schweizeri ( c ). MW—protein molecular weight marker (Thermo Scientific TM , Spectra TM Multicolor Broad Range Protein Ladder, Life Technologies Czech Republic Ltd., Prague, Czech Republic). Molecular masses (kDa) are indicated on the left side. FBG—human fibrinogen standard. The sample number is provided in the top line and hours since bite in the bottom line. Red arrows mark fibrinogen fragments induced by snakebite. The chains of human fibrinogen standards are highlighted with red square boxes.

Article Snippet: The FBG immunodetection was performed using rabbit anti-human fibrinogen polyclonal antibody (DAKO, HPST, Prague, Czech Republic; dilution 1:50,000 in 0.1% Tween/Tris-buffered saline) for 1 h at room temperature.

Techniques: Western Blot, SDS Page, Molecular Weight, Marker